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Full text of "USPTO Patents Application 10767200"

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CLAIMS 

1. An thod f producing an immunogen, which 
method comprises polymerising a hirudin and/ r a hirudin- 

5 like protein. r ' 

2 . a method according to claim 1 wherein the 
hirudin and/or the hirudin-like protein are/is polymerised 
with glutaraldehyde. 

3. a method according to claim 1 or 2 wherein the 
10 hirudin is HV1 (SEQ ID NO:l), HV2 %SEQ' ID NO: 2) r HV3 (SEQ 

ID NO:3>, PI (SEQ ID NO:4) or P2 (SEQ ID N0:5). 

4. a method according to claim; l or 2 wherein the 
hirudin-like protein is a derivative of J HVl (SEQ ID NO:l), 
HV2 (SEQ ID NO:2), HV3 (SEQ ID NO: 3} , PI (SEQ ID NO:4) or 

15 P2 (SEQ ID NO: 5) by way of amino acid substitution, 
deletion, insertion, extension, functiohalisation or 
chemical modification, said derivative having anti-thrombin 
activity. . •: , 

5. An immunogen comprising a polymerised hirudin 
20 and/ or hirudin-like protein. 

6. An immunogen according to claim 5 wherein the 
hirudin is HVl (SEQ ID NO: 1) , HV2 (SEQ ID N0:2) , HV3 (SEQ 
ID NO: 3), PI (SEQ ID N0:4) or P2 (SEQ ID NO: 5). 

7. An immunogen according to claim 5 wherein the 
25 hirudin-like protein is a derivative of HVl (SEQ ID N0:1), 

HV2 (SEQ ID NO: 2) , HV3 (SEQ ID NO: 3) / PI (SEQ ID NO:4) or' 
P2 (SEQ ID NO: 5) by way of amino acid substitution, • 
deletion, insertion, extension, functional isat ion or- 
chemical modification, said derivative having anti-tnrombin 
30 activity. ''■ . .£'•■;! . , 

8. An immunogen according to' claim 5, 6 or 7 
obtainable by polymerising the hirudin and/or the hirudin- 
like protein with glutaraldehyde. j 

9. A method of producing an antibody capable of 

35 binding to a hirudin or a hirudin-like protein, said method 
comprising raising antibody against an immunogen as claimed 



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in any one of claims 5 to 8. 

10. a method according to claim ^wherein th said 
antibody is a polycl nal antibody. .'".,.1 

11. Aithd according to claim ?5&erein the said 
5 antibody is a monoclonal antibody. 

12. a method for the quantitative determination, 
purification or identification of a hirudin or a hirudin- 
like protein, said method comprising raising antibody 
against an immunbgen as claimed 'in any one of claims 5 to 

10 8, and using the antibody thereby obtained in said 

quantitative determination, purification or identification.